it is my pleasure to invite you to the next Nencki Institute Seminar on Thursday May 12th at 3 pm, in the CN lecture hall. We will host dr Salvatore Spicuglia, head of the Functional genomics of normal and leukemic T cells team at the TAGC (Theories and Approaches of Genomic Complexity) in Marseilles.
Dr. Spicuglia will give a lecture entitled: Dissection of cis-regulatory elements with dual promoter and enhancer function: From high-throughput approaches to mechanistic insights
Gene expression is controlled by the involvement of gene-proximal (promoters) and distal (enhancers) regulatory elements. Our previous results have demonstrated that a subset of gene promoters, termed Epromoters, works as bona fide enhancers and regulates distal gene expression. Here, we hypothesized that Epromoters play a key role in the coordination of rapid gene induction during the inflammatory response. Using a high-throughput reporter assay we explored the function of Epromoters in response to type I interferon. We found that clusters of IFNa-induced genes are frequently associated with Epromoters and that these regulatory elements preferentially recruit the STAT1/2 and IRF transcription factors and distally regulate the activation of interferon-response genes. Based on these findings, we developed a bioinformatic pipeline to predict Epromoter-like regulated clusters. Using this pipeline, we identified and validated the involvement of Epromoter-containing clusters in the regulation of several inflammatory and stress responses. Our findings suggest that Epromoters function as a local hub recruiting the key TFs required for coordinated regulation of a subset of gene clusters during the inflammatory and stress responses.
We also cordially invite you to the get together reception which will follow dr Spicuglia’s talk.