On Thursday (03.07) we will host Prof. Young-Tae Chang. His lecture will be entitled: "Discrimination of live cells and reverse aging through selective removal of the senescent cells". The lecture will take place at 3 p.m. in the CN lecture hall and it will be followed by a get together.

Abstract

Cellular senescence and age-related dysfunction represent critical research areas in modern medicine with significant implications for understanding pathophysiology and developing therapeutic interventions. We present a novel, comprehensive fluorescent probe platform specifically engineered for high-resolution, real-time visualization and differentiation of cellular aging phenotypes across multiple dimensions of cellular biology. Our system integrates five complementary probe categories that collectively enable unprecedented discrimination of cell types and physiological states: Protein Oriented Live-cell Distinction (POLD) for protein-based phenotyping; Carbohydrate Oriented Live-cell Distinction (COLD) targeting glycomic signatures; Gating Oriented Live-cell Distinction (GOLD) for population-specific identification; Lipid Oriented Live-cell Distinction (LOLD) for membrane and organelle characterization; and Metabolism Oriented Live-cell Distinction (MOLD) for metabolic profiling. The platform offers significant advantages for clinical research applications, including compatibility with standard flow cytometry and microscopy techniques, minimal cytotoxicity, and rapid sample processing time. Additionally, the system demonstrates excellent specificity for discriminating between chronologically aged cells versus disease-induced premature senescence phenotypes, potentially aiding differential diagnosis. These advances represent a substantial improvement over existing methodologies for investigating cellular aging mechanisms and could facilitate development of more targeted therapeutic strategies for age-related pathologies.