The BD FACSAria I™Cell Sorter, which the Laboratory of Cytometry is equipped with, was upgraded to BD FACSAria IIu system. It is a first easy-to-use benchtop cytometer which delivers high-speed sorting and multicolor analysis. It is equipped with a fixed-alignment flow cell cuvette which provides superior fluorescence sensitivity. It can simultaneously detect up to 11 parameters (2 scatter signals and up to 9 fluorescent parameters) by utilizing 3 air-cooled, solid state lasers (available laser outputs at 405nm, 488nm and 633nm) and a fixed optical alignment system allowing for multiple configurations and a wide range of fluorochromes. The FACSAria allows for digital acquisition rates of up to 70,000 cells per second. The BD FACSAria is able to sort into two- and four-way bulk sorting devices for a variety of tube sizes (micro-tubes, 12 x 75ml test tubes, 15ml falcon tubes). The cytometer can sort using Automated Cell Deposition Unit (ACDU) to multi-well plates or on the microscope slide. The sorter is equipped with an Aerosol Management System that evacuates any aerosols from the sort chamber and has a refrigeration system that allows cooling of the sort sample. The FACSAria workstation is equipped with BD FACSDiva software that is used for the collection, storage, and analysis of digital data.