Research profile

Our research focuses on molecular mechanisms on molecular oncology and on gliomagenesis in particular. We apply next-generation sequencing methods, including transcriptomics, genomics and epigenomics to decipher molecular mechanisms behind complex mechanisms, like tumor formation or tumor progression or recurrence. The Laboratory conducts its own research and provides the core-facility services for investigators from the Nencki Institute and other scientific and R&D institutions.

Current research activities

– REST transcription factor role in cancer progression in IDH-mutant and IDH-WT gliomas

– DNA methylation role in shaping epigenomic environment of adult and pediatric gliomas

– Genomic and transcriptomic signatures of rare cancers

Selected publications

Stępniak K, Machnicka MA, Mieczkowski J, Macioszek A, Wojtaś B, Gielniewski B, Poleszak K, Perycz M, Król SK, Guzik R, Dąbrowski MJ, Dramiński M, Jardanowska M, Grabowicz I, Dziedzic A, Kranas H, Sienkiewicz K, Diamanti K, Kotulska K, Grajkowska W, Roszkowski M, Czernicki T, Marchel A, Komorowski J, Kaminska B, Wilczyński B. Mapping chromatin accessibility and active regulatory elements reveals pathological mechanisms in human gliomas Nat Commun. 2021 Jun 15;12(1):3621.

Dabrowski MJ#, Draminski M, Diamanti K, Stepniak K, Mozolewska MA, Teisseyre P, Koronacki J, Komorowski J, Kaminska B, Wojtas B.# Unveiling new interdependencies between significant DNA methylation sites, gene expression profiles and glioma patients survival Sci Rep. 2018 Mar 13;8(1):4390.

Wojtas B, Gielniewski B, Wojnicki K, Maleszewska M, Mondal SS, Nauman P, Grajkowska W, Glass R, Schüller U, Herold-Mende C, Kaminska B. Gliosarcoma Is Driven by Alterations in PI3K/Akt, RAS/MAPK Pathways and Characterized by Collagen Gene Expression Signature Cancers (Basel). 2019 Feb 27;11(3):284.

Roura AJ, Gielniewski B, Pilanc P, Szadkowska P, Maleszewska M, Krol SK, Czepko R, Kaspera W, Wojtas B#, Kaminska B Identification of the immune gene expression signature associated with recurrence of high-grade gliomas Journal of Molecular Medicine 2020

Król SK, Kaczmarczyk A, Wojnicki K, Wojtas B, Gielniewski B, Grajkowska W, Kotulska K, Szczylik C, Czepko R, Banach M, Kaspera W, Szopa W, Marchel A, Czernicki T, Kaminska B. Aberrantly Expressed RECQL4 Helicase Supports Proliferation and Drug Resistance of Human Glioma Cells and Glioma Stem Cells

Łastowska M, Trubicka J, Sobocińska A, Wojtas B, Niemira M, Szałkowska A, Krętowski A, Karkucińska-Więckowska A, Kaleta M, Ejmont M, Perek-Polnik M, Dembowska-Bagińska B, Grajkowska W, Matyja E. Molecular identification of CNS NB-FOXR2, CNS EFT-CIC, CNS HGNET-MN1 and CNS HGNET-BCOR pediatric brain tumors using tumor-specific signature genes Acta Neuropathol Commun. 2020 Jul 10;8(1):105.

Dabrowski MJ#, Wojtas B.# Global DNA Methylation Patterns in Human Gliomas and Their Interplay with Other Epigenetic Modifications Int J Mol Sci. 2019 Jul 15;20(14):3478.

Attachments

General principles of operation of the Sequencing Laboratory