On July 9th, 2019, the Polish Patent Office granted a patent for the invention entitled „Device for imaging of the transparent objects” (application no. P. 423173). The Patent inventors are Mr Łukasz Bożycki from the Laboratory of Biochemistry of Lipids, Mr Kacper Łukasiewicz from the Laboratory of Molecular Basis of Behavior and Mr Paweł Matryba from Laboratory of Neurobiology.
Tissue optical clearing is a method that renders it transparent, thus allows for the unobstructed penetration of the laser light through the entire specimen. In practice, it opens new avenue for imaging of the entire both isolated organs and clarified bodies of laboratory rodents. This, in turn, might facilitate observation of their structural modifications in ample scenarios or detection of pathological tissues, such as calcifications or tumors.
The subject matter of the patented invention is development of the light sheet microscopy-based device that allows for precise, high-throughput imaging of transparent organs and rodent bodies. Moreover, the device was combined with polarizers that allow rapid, unobstructed detection of tissue parts characterized by a distinct optical density.