Service Category: Flow cytometry

Cytometric data analysis

08.09.2020

Cytometric data analysis using various programs: FlowJo - allows the analysis and visualization of cytometric data, an additional algorithm tSNE enables the graphic representation of heterogeneous cell’s populations based on multi-parameter cytometric detection, ModFit- allows to analyse the cell cycle and differentiate cells’ populations based on phases.

Cell viability and apoptosis analysis

08.09.2020

Cell viability is measured as the percentage of living, healthy cells in a population. Cell viability tests are used to determine the general health status of cells, to optimize cell culture or ...

Cell cycle analysis

08.09.2020

Cell cycle analysis is a very popular application of flow cytometry. Utilization of the DNA-specific staining allows you to determine a DNA profile, thus e.g. the percentage of cell populations ...

Analysis of mitochondrial membrane potential

08.09.2020

Analysis using cationic lipophilic dyes such as JC-1, TMRE (tetramethyl rhodamine ethyl ester), DiOC6 (3), DiIC1 (5), CMXRos (MitoTracker Red), LDS-751, and rhodamine 123, which localize ...

Cytometric analysis of intracellular signaling pathways (phosphospecific flow cytometry)

08.09.2020

Cytometric method that is suitable for the intracellular signalling pathways analysis. It allows to distinguish different cell types based on surface markers and at the same time enables analysis ...