The laboratory possesses two FACSCaliburs, which are benchtop flow cytometers that provide standard multicolor analysis. They are equipped with two lasers, an air-cooled argon laser (488nm) and a red diode laser (635nm), which are spatially separated for high sensitivity needed for multicolor analysis. Up to four colors can be analyzed on this system using the 488 and 635 lasers. The detectors available from the 488 laser are 530/30, 585/42, >670, and from the 635 laser, 661/16. The BD FACSCalibur flow cytometer provides flexibility to support a wide variety of research such as cell analysis, assay development, verification, and isolation of cellular populations of interest.