The Revvity Opera Phenix Plus is an automated High Content Screening system that combines confocal microscopy with advanced image analysis, designed for fast, large‑scale studies of cell samples, organoids, and 3D cultures. It provides high resolution, spatial imaging, and multichannel fluorescence detection at high throughput, enabling simultaneous quantitative analysis of morphology, protein localization, and cellular responses. The integrated liquid‑handling module allows automated reagent dispensing and real‑time fast‑response experiments, further increasing reproducibility and accelerating screening workflows and pharmacological testing.