Analysis of internal and external membrane proteins (immunocytochemistry labeling)
The cytometric method used in the analysis of different intracellular molecules, utilizes intracellular staining protocols. To label intracellular antigens, the cells are fixed and then permeabilized before adding a detection antibody. This fixation/permeabilization procedure allows the antibody to enter the cell through the cell membrane without losing its morphological characteristics.
Milena Wiech, PhD