A fluorescent microscope consisting of an inverted, wide-field Leica DMI6000 microscope, with a motorized stage and an environmental chamber with the temperature and CO2 concentration controller. Equipped with a fluorescent lamp and ANDOR DU8285_VP and LEICA DCF 35DFXR2 cameras for detection. The microscope enables detection of fluorescent dyes with the emission range of 405 - 700 nm (fluo filters). A fast filter wheel allows ratiometric measurement of calcium concentration changes (Fura2). In addition, the microscope enables cell adhesion analysis using IRM (interference microscopy) and contrast methods: DIC and polarization. It is possible to image the whole specimen during one scan. Ideal for several days of in vivo experiments as it is possible to image up to several dozen different fields of view in one experiment.