The FEMTO3D ATLAS Plug&Play Dichro is an advanced plug‑and‑play two‑photon microscope designed for imaging neuronal activity in deep tissue layers and in the brain in vivo. The system uses an acousto‑optic scanner to enable fast 3D scanning across multiple regions simultaneously and to record calcium signals from large neuronal populations with high temporal resolution. It provides stable, long‑term functional imaging and is particularly suited for studies of neuronal networks, behavior, and neurophysiology.